Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (4): 198-202.doi: 10.3969/j.issn.1004-616x.1998.04.002

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CLONING AND IDENTIFICATION OF HUMAN CYTOCHROME P450 2C9 cDNA AND CONSTRUCTION OF ITS MAMMALIAN CELL EXPRESSION PLASMID

Wang Aijin , Qian Ying , Qian Yuli , and Yu Yingnian   

  1. Department of Pathophysiology and L aboratory of Medical Molecular Biology , Zhejiang Medical University , Hangz hou 310031
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-07-30 Published:1998-07-30

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Abstract: With reverse t ranscription polymerase chain reaction (RT2PCR) and DNA recombinant technique , a full2length cDNA encoding the monooxygenase cytochrome P450 2C9 from human liver was cloned into a plasmid p GEM23Z. The cDNA segment was analyzed by rest riction enzyme mapping and DNA sequencing , and was confirmed to be nothing other than CYP 2C9 cDNA. Then the CYP 2C9 cDNA was subcloned into a mammalian expression vector , ready for development of the cell line expressing CYP 2C9.

Key words: Human cytochrome P450, cDNA Cloning, RT2PCR, T2A cloning